Oct 19, 20 chromatographic are used extensively in organic chemistry laboratories for routines analysis. In this lab, you will perform thin layer chromatography tlc to identify an unknown drug and then use column chromatography to separate out the components of. Thin layer chromatography, or tlc, is a method for analyzing mixtures by separating the compounds in the mixture. Apr 11, 20 thin layer chromatography introduction thin layer chromatography or tlc is a technique used as a separation and identification technique. Excedrin is an overthecounter pain reliever that contains three analgesic components. Please turn in the answers to these questions when you arrive to your first lab. As the reaction proceeds, the solid salicylic acid disappears and the acetylsalicylic acid product. Tlc analysis of analgesic drugs aim of the experiment determine the composition of a series of.
It may be performed on the analytical scale as a means of monitoring the progress of a reaction, or on the preparative scale to purify small amounts of a compound. Overthecounter otc analgesics typically contain one or more of the following active. Read all of the required reading at the beginning of the experiment, especially analgesics p. All of them should be written in paragraphs and the. May 11, 2014 hi,this experiment is abouttlc analysis of analgesic drugsand i would like from you to write a report. Analyzing analgesics by thin layer chromatography tlc. Nonnarcotic or nonopioid nsaid,s and aspirin like analgesics. Tlc key thinlayer chromatography lab complete answer key. In thinlayer chromatography, the stationary phase is a polar absorbent, usually finely ground alumina or silica particles. This absorbent is coated on a glass slide or plastic sheet creating a thin layer of the particular stationary phase. Hplc method for the analysis of paracetamol, ca eine and.
Application of tlc densitometry method for simultaneous. E29 preparation of aspirin acetylsalicylic acid and thin. They are distinct from anesthetics, which temporarily affect, and in some instances completely eliminate, sensation. Hi,this experiment is abouttlc analysis of analgesic drugsand i would like from you to write a report. Your drug may contain from one to three of these substances. Essay about tlc analysis of analgesic drugs 7 words cram. Experiment 12 tlc analysis of analgesic drugs thin layer. Chromatography introduction column chromatography will be used to separate the components of extrastrength excedrin, or a generic of the same composition, a combination pain reliever that contains three active ingredients. Thin layer chromotography tlc can be used to determine the purity of compound, to analyze the composition of a mixture are differentiated by exposing to two competing phases, the stationary phase is a polar adsorbent such as silica gel or alumina, which has been coated on plastic plate. E29 preparation of aspirin acetylsalicylic acid and thinlayer chromatography of analgesic drugs the task to prepare a pharmaceutical compound and test its purity. Separation of the components of an analgesic tablet ii.
To assess the purity of the colored organic compounds provided to you, using tlc. Spot one plate with your 4 known standards acetaminophen, aspirin, caffeine, and ibuprofen and the other plate with the 5 unknown. In combination with nonsteroidal antiinflammatory drugs nsaids and opioid analgesics, paracetamol is used also in the management of severe pain such as. Elgebalyc aanalytical chemistry department, faculty of pharmacy, benisuef university,62111 benisuef, egypt. Have you taken the safety quiz on the chemsafety quiz sakai site. Pdf different spectrophotometric and tlcdensitometric. Analysis of drugs by thin layer chromatography experimental techniques required chromatography thinlayer t other documents. Six analgesics were spotted on the tlc plate including the unknown. Be able to deduce the relative polarity of organic compounds by comparing the functional groups. Thinlayer chromatography mohrig, 2nd edition, technique 15, pp. These include highpressure liquid chromatography hplc, gas chromatography gc, column chromatography, paper chromatography, and the technique you will be using in this experiment tlc. Jpc journal of planar chromatography modern tlc 2019, 32 6, 501504. Transfer the crushed tablet to a small test tube and add 1 ml of ethanol.
The unknowns behavior in thinlayer chromatography will be compared with that of its possible component analgesics. Analgesics are any compounddrug that provides relief from pain. An introductory laboratory on the identification of analgesics in an unknown sample. If you use any reference, please, write the complete. Pdf in organic chemistry, chromatography refers to the separation of a mixture or ions based. Method iv is tlc qr code for mobile users different spectrophotometric and tlc densitometric methods for determination of two analgesic drugs nada s. Pdf thin layer chromotography of analgesic drugs idayu.
Identification of overthecounter analgesics thinlayer chromatography tlc is a fast and simple method that can be used to identify organic molecules, verify the number of components in a mixture, or to monitor the. Thinlayer chromatography of analgesics introduction in this experiment, you will use thin. All of them should be written in paragraphs and the method should not be copied exactely from the instruction. Thin layer chromotography tlc can be used to determine the purity of compound, to analyze the composition of a mixture are differentiated by exposing to two. Thin layer chromatography lab report freebooksummary. Precut plastic backed or metal backed tlc plates, tlc capillaries, uv lamp aim the goal of your experiment is two fold. Aspirin, acetaminophen, and caffeine separation from. Essay about tlc analysis of analgesic drugs 625 words cram. Lab report on tlc analisys of analgestic drugs essay examples. An analgesic or painkiller is any member of the group of drugs used to achieve analgesia, relief from pain. Ideal for the first week of an organic chemistry sequence to get students familiar with their surroundings. Be careful with the spotting capillaries, they can be sharp be careful when making the spotting capillaries, glass heated in a flame is hot. A benefitrisk assessment of caffeine as an analgesic adjuvant. There are many different types of chromatographic techniques used today.
There are many forms of chromatography, but one thing that remains constant throughout all of the types of chromatography is. Salicylic acid is the substance that has analgesic, antiinflammatory, and antipyretic properties. From the array of components you discover by tlc, you will be able to identify the otc drug name from table 1. The skills by the end of the experiment you should be able to. May 03, 20 antipyretic, antiinflammatory and analgesic drugs. The combination of paracetamol with dipyrone is used as an antipyretic, analgesic and antiin. Thin layer chromatography introduction thin layer chromatography or tlc is a technique used as a separation and identification technique. Tlc analysis of analgesic drugs flashcards quizlet. Tlc analysis of analgesic drugslab report 1 thin layer. Chromatographic are used extensively in organic chemistry laboratories for routines analysis. Filling the need for an uptodate, complete reference, thin layer chromatography in drug analysis covers the most important methods in pharmaceutical applications of tlc, namely, analysis of bulk drug material and pharmaceutical formulations, degradation studies, analysis of biological samples, optimization of the separation of drug classes.
To determine the identities of the analgesic components present in an unknown mixture by comparison with known analgesic samples using tlc. Pdf chromatography analyzing analgesics by tlc and. You will be given five known reference substances, an unknown. In this experiment, thin layer chromatography tlc will be used to identify the active ingredients in some of these products. Thin layer chromatography tlc analysis of analgesic drugs. Individual variability in pain perception and differences in the efficacy of analgesic drugs are complex phenomena and are partly genetically predetermined. Experimental procedure, report template introduction in this experiment, thin layer chromatography tlc will be used to examine the composition of various analgesic painrelieving drugs by comparison with the. Analgesic drugs act in various ways on the peripheral and central nervous systems. Acetaminophen, acetylsalicylic acid, caffeine, and ibuprofen.
Essentially, tlc has many purposes within the setting of lab. Introduction thin layer chromatography also known as tlc is the physical separation of a mixture into its individual components by distributing the components between a stationary phase the porous tlc plate and a mobile phase the solvent that moves through the stationary phase and carries the material that needs. Thin layer chromotography of analgesic drugs objectives. Method iv is tlc qr code for mobile users different spectrophotometric and tlcdensitometric methods for determination of two analgesic drugs nada s. Learn about how chemicals can be separated based on polarity through thin layer chromatography tlc. Thin layer chromatography analysis of analgesic drugs essay. Thin layer chromatography of the unknown analgesic jessica bajao, phoebe abalos, kevin antiga, carmelus aseneta 3biology 2 college of science, university of santo tomas, manila, philippines abstract the group used five different analgesics in this experiment. Thin layer chromatography analysis of analgesic drugs essay sample. Learn vocabulary, terms, and more with flashcards, games, and other study tools. In this experiment, you will use tlc to identify unknown analgesic painkillers using the table of analgesics and their components in the experimental section of this experiment. Tlc can be used to help determine the number of components in a mixture, the identity of compounds, and the purity of a compound. Thin layer chromatography tlc is a method for analyzing mixtures by separating the.
Tlc chromatographicdensitometric assay of ibuprofen and its. Thin layer chromatography in drug analysis crc press book. Almost all mixtures of solvents can be used as the mobile phase. Thin layer chromatography rev 22017 thin layer chromatography tlc is a method for analyzing mixtures by separating the compounds in the mixture based on polarity. Identification of overthecounter analgesics thinlayer chromatography tlc is a fast and simple method that can be used to identify organic molecules, verify the number of components in a mixture, or to monitor the progress of a reaction.
January 24, 20 tlc analysis of analgesic drugs introduction. The use of thin layer chromatography in the examination of. Objective the purpose of this experiment is to identify an unknown proprietary drug using thinlayer chromatography. Draw a light pencil line about 1 cm from the end of each chromatographic plate. Lab report on tlc analisys of analgestic drugs essay examples 1266 words 6 pages tlc analysis of analgesic drugs introduction in this experiment, thinlayer chromatography tlc was used to determine the composition of various overthecounter otc analgesics. Tlc analysis of analgesic drugslab report 1 free download as word doc. Solved experiment 3 tlc analysis of analgesic drugs. Visualization and analysis when the tlc plate is dry, observe the spots under shortwavelength 254 nm ultraviolet light, outline them with a. Numerous methods have been reported for the analysis of paracetamol and its combinations in pharmaceuticals or in biological. However, the analgesic adjuvant effects of caffeine have not been seriously investigated since a pooled analysis conducted in 1984 showed that caffeine reduces the amount of paracetamol acetaminophen necessary for the same effect by approximately 40%. Identification of analgesic drugs tlc cerritos college. Overthecounter otc analgesics typically contain one or more of the following active ingredients. Tlc analysis of analgesics chem 21l unc charlotte studocu.
Different spectrophotometric and tlcdensitometric methods. The possible unknowns and their analgesic ingredients will be anacin aspirin, caffeine, excedrin acetaminophen, caffeine, aspirin, motrin. Aspirin, acetaminophen, and caffeine separation from excedrin via column chromatography. Design of a drug discovery course for nonscience majors. Experiment 12 tlc analysis of analgesic drugs free download as word doc. Learning objectives understand the basic principles of polarity of organic compounds. Tlc analysis of analgesic drugs introduction in this experiment, thinlayer chromatography tlc was used to determine the composition of various overthecounter otc analgesics. In this lab the objective was to determine the active ingredients. A method for the thinlayer chromatography of analgesic. In this experiment, thin layer chromatography tlc will be used to examine the composition of various analgesic painrelieving drugs by comparison with the. Thin layer chromatography of the unknown analgesic free. The report should be up to 800 words and it should include. Students extract, isolate, and perform thin layer chromatography on aspirin, acetominophen, or ibuprofen. In this experiment, a thinlayer chromatography tlc was used to determine the composition of various overthecounter analgesics.
Thinlayer chromatography analysis of analgesics adam cap. Different spectrophotometric and tlcdensitometric methods for determination of two analgesic drugs article pdf available april 2014 with 55 reads how we measure reads. Caffeine has been an additive in analgesics for many years. The purpose of this experiment is to focus on the ingredients that the analgesic drugs may contain.